microRNA Analysis Service
microRNAs (miRNAs) are small non-coding RNA molecules that play pivotal roles in post-transcriptional gene regulation. By binding to target mRNAs, miRNAs induce translational repression or mRNA degradation, influencing diverse biological processes including development, differentiation, and disease progression. CD ComputaBio's microRNA analysis service leverages advanced transcriptomics technologies to deliver precise, high-throughput miRNA profiling and functional characterization.
Introduction to microRNA Analysis
microRNAs are short, single-stranded RNA molecules, typically around 22 nucleotides in length. They exert their regulatory effects by binding to complementary sequences within the 3' untranslated regions (3' UTRs) of target messenger RNAs (mRNAs). This binding interaction can result in either translational repression or mRNA degradation, effectively modulating the expression levels of target genes. Given that a single miRNA can target multiple mRNAs and multiple miRNAs can act on the same mRNA, miRNAs are integral components of intricate gene regulatory networks.
Figure 1. microRNA Analysis. (Siddika T, et al.,2021)
Our Services
CD ComputaBio offers a suite of specialized microRNA analysis services tailored to meet the diverse needs of researchers.
miRNA Expression Profiling
In miRNA expression profiling, we use high-throughput sequencing and microarray technologies. Sequencing offers a detailed look at the miRNA transcriptome, detecting known and novel miRNAs and quantifying their expression. Microarrays, meanwhile, quickly and cost-effectively compare miRNA profiles across samples. The data helps identify differentially expressed miRNAs, offering insights into their roles in biology and disease.
miRNA Target Prediction and Validation
For miRNA target prediction and validation, CD ComputaBio combines computational algorithms with experiments. We first predict miRNA targets based on sequence features. Then, we use luciferase reporter assays, Western blot, and quantitative PCR for validation, confirming miRNA-target interactions and their impact on gene expression.
miRNA-mRNA Interaction Analysis
miRNA-mRNA interaction analysis service integrates miRNA and mRNA expression data with interaction information. Through bioinformatics, we identify regulatory miRNA-mRNA pairs, construct interaction networks, and analyze regulatory patterns, giving a comprehensive view of miRNA-mediated gene regulation.
Analytical Methods in microRNA Analysis
qRT-PCR for miRNA
Due to miRNAs' short length, specialized primers and reverse transcription methods are needed. Stem-loop reverse transcription primers convert miRNAs to cDNAs, which are then amplified and quantified with specific primers. It reliably validates miRNA expression changes from high-throughput methods.
Microarray-based miRNA Analysis
miRNA microarrays have probes complementary to known miRNAs on a glass slide. Labeled miRNA samples hybridize to the array, and probe signal intensity indicates miRNA expression levels. Ideal for comparing profiles across samples and finding differentially expressed miRNAs.
Next-Generation Sequencing (NGS) for miRNA
NGS sequences the entire miRNA population, detecting known, novel miRNAs and modifications. Bioinformatics analysis of sequencing data identifies differentially expressed miRNAs, predicts targets, and studies regulatory networks. It excels at detecting low-abundance miRNAs overlooked by other methods.
Our Advantages
Cutting-Edge Technological Infrastructure
CD ComputaBio uses the latest high-throughput sequencing and microarray tech for precise, comprehensive microRNA data, ensuring reliable results for research.
Multifaceted Analytical Approach
By merging computational prediction with experimental validation, the service offers a 360-degree view of microRNA analysis. This combination boosts result reliability and uncover deeper regulatory mechanisms.
Expert-Driven Customizable Service
Staffed by skilled experts, the service delivers in-depth analysis and insights. It's fully customizable and offers fast turnaround, speeding up research without sacrificing quality.
CD ComputaBio's microRNA analysis service combines state-of-the-art transcriptomics, bioinformatics, and functional validation to accelerate discoveries in basic research and translational medicine. By decoding miRNA regulatory networks, researchers gain unprecedented insights into disease mechanisms and therapeutic opportunities. If you are interested in our services or have any questions, please feel free to contact us.
Reference:
- Siddika T, Heinemann I U. Bringing MicroRNAs to light: methods for MicroRNA quantification and visualization in live cells. Frontiers in bioengineering and biotechnology, 2021, 8: 619583.
* For Research Use Only.
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