Fusion protein is a group of proteins that mediate the fusion of the two plasma membranes, as one of the two glycoproteins contained in the outer leaflet of the viral lipid bilayer mediates the fusion of the viral envelope with the host cell plasma membrane. Another glycoprotein is hemagglutinin ceramidase. Two different proteins can be linked into a large molecule, either chemically or by the fusion of genes. Figure shows the fusion of two genes (BCR-ABL) to encode a recombinant oncogenic protein.
Step 1: Determine the vectors.
Step 2: Make sure the necessary conditions for plasmid expression vectors are met, including selection markers, origins of replication, promoters, start codons, multiple cloning sites, and transcription terminators.
Step 3: The T7 promoter system is a commonly used expression system, containing the T7 promoter, ribosome binding site, start codon, multiple cloning site and transcription terminator.
Step 4: The RNA polymerase of the T7 promoter is expressed in the genome of a special strain. The polymerase gene is integrated into the bacterial genome in the phage lysogen pathway and induced by IPTG.
Step 5: After constructing the fusion protein gene on the expression vector, it is transferred into the host bacteria, and the fusion protein is expressed under the induction of IPTG.
Figure 2. Workflow for predicting the structure of fusion proteins.
|Project name||Fusion protein modeling|
|Samples requirement||Our fusion protein modeling requires you to provide specific requirements.|
|Timeline||Decide according to your needs.|
|Deliverables||We provide you with raw data and modeling results.|
CD ComputaBio has rich experience in fusion protein modeling technology. The models we obtain have been quality verified and can be used for downstream calculation simulations. Our expert team can provide accurate modeling services for the system you choose, so you don't have to worry about technical issues. If you need fusion protein modeling services, please feel free to contact us.
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