Fluorescence belongs to the emission spectrum. The compound absorbs energy to transition The compound absorbs energy to transition from the ground state to the excited state. It then releases energy in a non-radiative manner. Relax to the minimum point of excited state energy (excited state singlet state, excited state minimum point conformation), and then de-excited to the ground state (ground state singlet state, excited state minimum point conformation) by releasing photons (emitting fluorescence) ), as shown in Figure 1.
In fact, the above process can be rephrased in Figure 2:
Fluorescence is a process of de-excitation from the minimum point of excited-state energy to the ground state, so the fluorescence wavelength can be predicted by finding the minimum point of excited-state energy.
As shown in Figure 2, the compound releases energy in the form of photons when de-excited from the excited singlet state S1 to the ground state singlet state S0. Assuming that this energy is all converted into photons, the wavelength of the photon corresponds to the emitted fluorescence wavelength. In this process, S1 and S0 have exactly the same conformation, and the energy difference between the two is the wavelength.
|Project name||Fluorescence Spectrum Prediction Service|
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