Nucleotide Analysis Service
Nucleotides, as DNA/RNA building blocks, are essential for genetic coding, cellular signaling, and metabolism. Transcriptomic advances now enable detailed study of their roles in gene regulation and disease. CD ComputaBio's nucleotide analysis service combines metabolomics and bioinformatics to analyze nucleotide variations, modifications, and pathways, supporting genomics research and therapeutic development.
Introduction to Nucleotide Analysis
Nucleotides form DNA/RNA's basic structure, composed of a nitrogenous base and phosphate group. Their sequences encode genetic instructions for all life processes. In transcriptomics, nucleotide analysis examines RNA to determine:
- Gene expression levels and patterns
- Alternative splicing events producing protein variants
- Non-coding RNAs with regulatory functions
Through sequencing and bioinformatics, this analysis reveals cellular regulatory networks and molecular mechanisms underlying biological processes.
Figure 1. Nucleotide.
Our Services
Nucleotide analysis is a multifaceted field, and CD ComputaBio offers a suite of comprehensive services tailored to meet the diverse needs of researchers.
Nucleotide-Metabolite Profiling
CD ComputaBio leverages high-end analytical platforms like high-resolution LC-MS for nucleotide-metabolite profiling. This method accurately identifies and quantifies a broad range of nucleotide-derived metabolites, offering a comprehensive overview of the nucleotide metabolome. It helps detect metabolite level changes across various conditions, providing essential data for research on drug impacts, dietary effects, etc.
Pathway-Specific Nucleotide Analysis
Pathway-specific analysis at CD ComputaBio targets nucleotides and related metabolites within defined metabolic pathways. Using custom methods and databases, it precisely measures key nucleotide levels in processes such as purine and pyrimidine metabolism. This enables researchers to understand pathway mechanisms and identify potential therapeutic or biotech intervention points.
Comparative Nucleotide Metabolomics
CD ComputaBio's comparative service allows researchers to compare nucleotide metabolomes across different samples, including healthy and diseased tissues or samples from various developmental stages. Statistical analysis highlights significant metabolite level differences, aiding in biomarker discovery and understanding the molecular basis of phenotypic variations.
By Workflow
Sample Preparation: Extraction of nucleotides from biological matrices (cells, plasma, tissues) using optimized protocols to prevent degradation. Quality control (QC) samples are included to monitor technical variability.
Sample Analysis: Instrumental analysis in nucleotide analysis involves two key methods. Liquid Chromatography-Mass Spectrometry (LC - MS/MS) offers high sensitivity and selectivity.
Data Processing: Raw data preprocessing (peak alignment, noise reduction, normalization). Compound identification using in-house and public databases.
Report Delivery: Detailed analysis with visualizations such as heatmaps and pathway diagrams is accompanied by biological interpretation to offer actionable insights.
Our Advantages
Advanced Instrumentation: CD ComputaBio's service utilizes a diverse range of high-end analytical instruments. This ensures comprehensive identification and profiling of nucleotide metabolites.
Multidisciplinary Expertise: CD ComputaBio offers a holistic approach to nucleotide analysis. Their knowledge aids in experimental design, data interpretation, and overcoming research challenges.
Cost-effective Solutions: Our service provides flexible, tailored packages to suit different research requirements and budgets.
CD ComputaBio's nucleotide analysis service empowers researchers with precise, high-throughput metabolomic profiling to uncover the roles of nucleotides in health and disease. By leveraging advanced analytical platforms and bioinformatics expertise, the service delivers actionable data to accelerate discoveries in biomedicine, pharmacology, and biotechnology. If you are interested in our services or have any questions, please feel free to contact us.
* For Research Use Only.
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