Lipidomics Analysis Service
Exploring biological systems at multiple molecular levels is crucial. While transcriptomics reveals gene expression patterns, lipidomics analysis, as a complementary discipline, offers insights into cellular metabolism and processes. CD ComputaBio provides advanced lipidomics analysis services, enabling the study of lipid profiles and integration with transcriptomics for a holistic understanding of biology.
Introduction to Lipidomics Analysis
Lipidomics aims to comprehensively identify, quantify, and characterize the lipidome. Driven by mass spectrometry advancements, the process involves lipid extraction from various biological samples, followed by ionization, fragmentation, and detection in a mass spectrometer. High-resolution mass spectrometry enhances accuracy, and integrating lipidomics with transcriptomics uncovers the link between gene expression and lipid metabolism.
Figure 1. Lipidomics Analysis. (Li Z, et al., 2021)
Methods of Lipidomics Analysis
Targeted Lipidomics
Targeted lipidomics focuses on specific lipid molecules or categories. Based on known structures and metabolic pathways, it selects targets and uses highly sensitive and specific techniques like LC-MS/MS for precise quantitative and qualitative analysis. It zeroes in on the roles of specific lipids in biological processes and diseases, and is applicable to the study of known lipid functions and the screening of markers.
Untargeted Lipidomics
Untargeted lipidomics conducts a comprehensive and unbiased analysis of all lipids in biological samples without pre-setting targets. By using high-resolution mass spectrometry and advanced analysis methods, it widely detects, identifies, and relatively quantifies lipid molecules. It helps discover new lipids and metabolic pathways, reveals the overall picture of lipid metabolism.
Our Services
CD ComputaBio offers a diverse range of services to meet the specific needs of our clients. Our services are tailored to provide you with in-depth and actionable insights.
By Lipid Types
Phospholipid Analysis
Phospholipids, crucial for cell membrane structure and function, participate in membrane fluidity, signal transduction, and cell-cell recognition. CD ComputaBio's service comprehensively identifies and quantifies phospholipid classes like phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol.
Sphingolipid Analysis
Sphingolipids play diverse roles in cell signaling, adhesion, and immune regulation. CD ComputaBio's service precisely characterizes sphingolipid classes, including ceramides, sphingomyelins, and glycosphingolipids, using high-resolution mass spectrometry and targeted methods.
Neutral Lipid Analysis
Associated with energy storage and lipid homeostasis, neutral lipids such as triglycerides and sterols are analyzed by CD ComputaBio's service. It accurately quantifies these lipid classes, enabling studies on lipid accumulation, energy metabolism, and lipid-related diseases.
Other Lipid Analysis
Beyond phospholipid, sphingolipid, and neutral lipid analysis, CD ComputaBio provides analysis for various other lipid types, including Fatty Acids (FA), Glycerolipids (GL), Glycerophospholipids (GP), Sphingolipids (SP), Sterol Lipids (ST), Pregnenol Lipids (PR), and Polyketides (PK). Our services ensure accurate identification and quantification, supporting lipid - related research.
By Workflow
| Sample Collection |
Samples are collected following standardized procedures for consistency and immediately processed or stored properly to prevent lipid degradation. |
| Lipid Extraction and Preparation |
Appropriate extraction methods are selected based on sample types. After extraction, lipid extracts are dried and reconstituted for mass spectrometry analysis. |
| Sequencing |
Samples are sequenced with the read mode and read depth most suitable for your application. |
| Mass Spectrometry and Data Analysis |
Samples are analyzed in different mass spectrometry modes. Raw data is processed with specialized software for peak detection, identification, and quantification. Comprehensive reports present the final lipid profiles. |
Our Advantages
Proprietary Analytical Protocols
CD ComputaBio's proprietary analytical protocols, refined through extensive research, optimize lipid extraction and analysis. Tailored to diverse lipid types and biological matrices, these protocols ensure reliable, comprehensive data even for challenging samples, enabling high-quality research outcomes.
Global Data Integration Capabilities
CD ComputaBio excels in integrating lipidomics data with other omics datasets like transcriptomics, proteomics, and metabolomics. This holistic integration reveals molecular relationships, aids in identifying new biological pathways, and offers a comprehensive multi-omics understanding of biological systems.
Rapid Prototyping for Custom Research
For custom lipidomics projects, we provide rapid prototyping. The company quickly designs and executes customized experimental setups and analysis pipelines, reducing project timelines. This enables efficient hypothesis testing and research iteration, offering flexibility and speed for diverse research needs.
CD ComputaBio offers fast turnaround times for its lipidomics services without sacrificing data quality. Researchers can expect timely delivery of results, allowing them to progress their projects efficiently. Detailed and easy-to-understand reports are provided, along with technical support and consultation, to assist researchers in fully utilizing the lipidomics data for their scientific research. If you are interested in our services or have any questions, please feel free to contact us.
Reference:
- Li Z, Cheng S, Lin Q, et al. Single-cell lipidomics with high structural specificity by mass spectrometry. Nature communications, 2021, 12(1): 2869.
* For Research Use Only.
Related Services
