mRNA analysis is a cutting-edge transcriptomics service that enables comprehensive profiling of mRNA molecules to support drug discovery, vaccine development, and precision medicine. CD ComputaBio's high-throughput sequencing and bioinformatics solutions provide critical insights into gene expression, splice variants, and novel biomarkers, ensuring robust data for preclinical and clinical-stage research.
Introduction to mRNA Analysis
mRNA molecules serve as transient blueprints for protein synthesis, carrying genetic instructions from DNA to ribosomes. Their abundance and stability are tightly regulated, making mRNA levels a direct indicator of gene activity. Key aspects of mRNA biology include:
- Transcriptional Regulation: Influenced by transcription factors, epigenetic modifications, and signaling pathways.
- Post-Transcriptional Modifications: Alternative splicing, polyadenylation, and RNA editing generate transcript diversity.
- Degradation Dynamics: mRNA turnover rates impact protein expression and cellular responses.
Figure 1. mRNA Analysis. (Vanhinsbergh C J, et al., 2022)
Our Services
CD ComputaBio offers a suite of advanced mRNA analysis solutions tailored to diverse research needs. Key services include:
- Comprehensive mRNA Sequencing Analysis: From sample processing to result delivery, we offer one-stop services that cover RNA extraction, cDNA synthesis, high-throughput sequencing, and data analysis.
- Differential Expression Gene Analysis: By utilizing advanced statistical tools, we are able to identify genes that exhibit significant changes under different conditions, and further analyze their biological significance.
- Alternative Splicing Analysis: Detection and quantification of mRNA splice variants, providing insights into protein diversity and regulatory mechanisms.
- mRNA Stability and Turnover Studies: Examination of transcript half-lives and degradation patterns through specialized sequencing approaches.
Workflow of mRNA Analysis
Sample Preparation: RNA is extracted from samples (cells/tissues/biofluids) with strict quality control (RIN>8), followed by mRNA enrichment via rRNA depletion or poly-A selection.
Sequencing: Both high-depth short-read sequencing and isoform - resolving long-read sequencing methods are accessible.
Bioinformatics: Data processing includes genome alignment, expression quantification, and quality checks (PCA/sample correlation).
Reporting: Interactive reports with visualizations (heatmaps/volcano plots) and annotated results for clear interpretation.
Our Advantages
Multi-Platform Expertise
Our service offers unparalleled flexibility with multiple NGS platforms. Researchers benefit from our extensive experience in matching sequencing platforms to different study designs, whether requiring high-throughput, long-read, or specialized applications.
Single-Cell Resolution
High-sensitivity scRNA-seq services reveal intricate cellular heterogeneity and rare cell populations with exceptional accuracy. This cutting-edge approach enables researchers to uncover previously hidden transcriptional dynamics within complex tissues and biological systems.
Customized Bioinformatics
CD ComputaBio develops specialized analysis pipelines tailored to unique research needs, including novel organism studies and specialized applications. Our team of bioinformatics experts works closely with researchers to implement custom solutions that address specific analytical challenges.
CD ComputaBio's mRNA analysis service empowers researchers with precise, scalable, and interpretable transcriptomic data. From bulk RNA-seq to single-cell resolution, the service bridges the gap between gene expression and functional biology, accelerating discoveries in disease mechanisms, therapeutic development, and beyond. If you are interested in our services or have any questions, please feel free to contact us.
Reference:
- Vanhinsbergh C J, Criscuolo A, Sutton J N, et al. Characterization and sequence mapping of large RNA and mRNA therapeutics using mass spectrometry. Analytical Chemistry, 2022, 94(20): 7339-7349.
* For Research Use Only.
Related Services
